Development of salmon specific, immunology assays for automated analysis (RD156)

Project Outcome:

The project ‘Development of salmon specific, immunology assays for automated analysis’ has explored innovative tools to monitor the health of salmon through the detection of specific biomarkers, with a focus on the immune response. The primary objective was to enhance disease detection and overall health monitoring in aquaculture by utilising biomarkers such as IgM and troponins, which are key indicators of salmon health. The research faced challenges, including compatibility with key laboratory instruments, which prompted a shift from the originally planned turbidimetric assay. Despite these challenges, the project successfully developed alternative approaches, such as using nanogold sensors for biomarker detection with a plate-based approach followed by lateral flow tests, as well as multiplex immunoassays using Luminex technology. These innovations resulted in the creation of rapid and cost-effective diagnostic tools.

The findings from this work have led to the development of new collaborations and have advanced the understanding of salmon immune responses. The diagnostic tools established in this project will improve early disease detection and fish health management by enabling more precise monitoring of salmon health. In particular, the focus on biomarkers like IgM and troponins has contributed to the development of methods to detect bacterial and viral infections more effectively. These advancements are crucial for the sustainability of the seafood industry, as they support seafood security and help reduce the environmental impact of fish farming by improving disease management practices.